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Jim Endrizzi, Post doctoral fellowContact Information:Montana State UniversityDepartment of Chemistry and Biochemistry224 Chemistry and Biochemistry & BuildingBozeman, MT 59717
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Research Interests
After 12.3 years of post-graduate research at the University of California, I finally made it to the Peters' lab in Bozeman, MT. My primary focus is to establish structure-based catalytic mechanisms for bacterial carbon-fixing enzymes. The enzymes I am working with function in metabolism of propylene, propane and isopropanol in aerobic soil bacterium Xanthobacter (strain Py2).
I am currently building an x-ray crystallographic model of a 370 kDa hexamic Acetone Carboxylase (AC) comprised of 3 subunits. We aim to identify the novel mechanism of CO2 fixation employed by AC, as it requires no special cofactors other than ATP and Mn2+. With 20 years of experience in structural biology, I also enjoy assisting other researchers with particularly vexing crystallographic problems.
Outside the lab, I like to run, mountain bike, ski (water, cross-country, & downhill), swim, rope swing, cliff dive, roller blade, camp, hike, raft, sauna, hot spring, and cook. I also enjoy live music and avoiding potential professorships. My hobbies include x-ray crystallography, molecular biology and mechanistic enzymology.
Education and Research Experience
1986 1986-1987 1993
1994-1998 1998-2002
2002-2006
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B.S. in Chemistry, University of Minnesota, Duluth, MN, Minors: Biology, Mathematics, Psychology Bicycled ~ 30,000 miles researching graduate schools, among other distractions Ph.D. in Chemistry, Institute of Molecular Biology, University of Oregon, Eugene, OR (PI: Dr. S.J. Remington and collaboration with Carlsberg labs (makers of elephant beer, Copenhagen) Post Doctoral Researcher, University of California, Berkeley, CA (PI: Dr. T. Alber) Research Associate Specialist, University of California, Berkeley, CA (PI's: Dr. T. Alber, Dr. H.K. Schachman) Research Associate Specialist, University of California, Davis, CA (PI: Dr. Enoch P. Baldwin)
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Awards and Honors
1993
1982-1986
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The Crystal Duck Award, presented by Dr. Bob Stroud, for Creativity and "Maturity" in Science (Huh? What did Bob mean by the quotation marks??) West Coast Protein Crystallography Workshop, Asilomar, CA Tozer Scholarship |
Publications
Krishnakumar, AM; Sliwa, D; Endrizzi, JA; Boyd, ES; Ensign, SA; Peters, JW. Getting a handle on the role of coenzyme M in alkene metabolism. Microbiol. Mol. Biol. Rev.; 72: 445-456.
Cronk, JD; Rowlett, RS; Zhang, KY; Tu, C; Endrizzi, JA; Lee, J; Gareiss, PC; Preiss, JR. Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase. Biochemistry 2006; 45: 4351-61.
Endrizzi, JA; Kim, H; Anderson, PM; Baldwin, EP. Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex. Biochemistry 2005; 44: 13491-9.
Endrizzi, JA; Kim, H; Anderson, PM; Baldwin, EP. Crystal structure of Escherichia coli cytidine triphosphate synthetase, a nucleotide-regulated glutamine amidotransferase/ATP-dependent amidoligase fusion protein and homologue of anticancer and antiparasitic drug targets. Biochemistry 2004; 43: 6447-63.
Young, TA; Delagoutte, B; Endrizzi, JA; Falick, AM; Alber, T. Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases. Nat Struct Biol 2003; 10: 168-74.
Cronk, JD; Endrizzi, JA; Cronk, MR; O'Neill J, W; Zhang, KY. Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity. Protein Sci 2001; 10: 911-22.
Rose, RB; Endrizzi, JA; Cronk, JD; Holton, J; Alber, T. High-resolution structure of the HNF-1alpha dimerization domain. Biochemistry 2000; 39: 15062-70.
Rose, RB; Bayle, JH; Endrizzi, JA; Cronk, JD; Crabtree, GR; Alber, T. Structural basis of dimerization, coactivator recognition and MODY3 mutations in HNF-1alpha. Nat Struct Biol 2000; 7: 744-8.
Howard, BR; Endrizzi, JA; Remington, SJ. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 A resolution: mechanistic implications. Biochemistry 2000; 39: 3156-68.
Endrizzi, JA; Beernink, PT; Alber, T; Schachman, HK. Binding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: implications for allosteric regulation. Proc Natl Acad Sci USA 2000; 97: 5077-82.
Cronk, JD; O'Neill, JW; Cronk, MR; Endrizzi, JA; Zhang, KY. Cloning, crystallization and preliminary characterization of a beta-carbonic anhydrase from Escherichia coli. Acta Crystallogr D Biol Crystallogr 2000; 56: 1176-9.
Beernink, PT; Endrizzi, JA; Alber, T; Schachman, HK. Assessment of the allosteric mechanism of aspartate transcarbamoylase based on the crystalline structure of the unregulated catalytic subunit. Proc Natl Acad Sci USA 1999; 96: 5388-93.
Endrizzi, JA; Zhang, G; Xiong, WC; Hurley, JH; Remington, SJ; Colman, RF. Crystallization and preliminary diffraction analysis of porcine heart mitochondrial NADP(+)-dependent isocitrate dehydrogenase. Acta Crystallogr D Biol Crystallogr 1996; 52: 1024-6.
Cronk, JD; Endrizzi, JA; Alber, T. High-resolution structures of the bifunctional enzyme and transcriptional coactivator DCoH and its complex with a product analogue. Protein Sci 1996; 5: 1963-72.
Endrizzi, JA; Cronk, JD; Wang, W; Crabtree, GR; Alber, T. Crystal structure of DCoH, a bifunctional, protein-binding transcriptional coactivator. Science 1995; 268: 556-9.
Endrizzi, JA; Breddam, K; Remington, SJ. 2.8-A structure of yeast serine carboxypeptidase. Biochemistry 1994; 33: 11106-20.
Svendsen, I; Hofmann, T; Endrizzi, JA; Remington, SJ; Breddam, K, The primary structure of carboxypeptidase S1 from Penicillium janthinellum. FEBS Lett 1993; 333: 39-43.
Anderson, PM; Johnson, WV; Endrizzi, JA; Little, RM; Korte, JJ. Interaction of mono- and dianions with cyanase: evidence for apparent half-site binding. Biochemistry 1987; 26: 3938-43.
