Biochemistry, Biological Signaling Mechanisms and Global Proteomic Analysis
Office: Room251 Chemistry and Biochemistry Building
Lab: Room 244 Chemistry and Biochemistry Building
P.O. Box 173400
Bozeman, MT 59717
B.A., Carleton College, 1961; Ph.D., University of California at Berkeley, 1966; Postdoctoral, University of California, Berkeley, 1966-67; Postdoctoral, Massachusetts Institute of Technology, 1967-69.
· BCH 104RN THE BIOCHEMISTRY OF HEALTH FOR NON-SCIENCE MAJORS
· BCH 442 METABOLIC REGULATION
Awards and Professional Activities:
Helen Hay Whitney Postdoctoral Fellow, 1966-69; NATO International Travel Fellow, 1975-80; European Molecular Biology Organization Senior Fellow, 1981-83; Charles and Nora Wiley Award for Meritorious Research and Creativity, 1995; Sabbatical Fellowship to Harvard Medical School and MIT, 1996-97 and to the National Proteomics Center at the Medical College of Wisconsin 2003-4.
The Dratz lab uses global proteomic analysis to investigate signaling networks in cells and studies the structure and function of membrane receptor and amplifier proteins. The genome of an organism is quite static (apart from rare mutations), whereas the proteins expressed or modified by cells often change rapidly in response to stimuli. Functional proteomics is the study of the proteins that are up- or down-regulated, or change in post-translational modifications (PTMs), in response to biological or environmental stimuli. Proteins typically contain many different PTMs, which affect the protein’s activity, cellular localization, or protein partners. Proteomics technology uses mass spectrometers for protein identification and for characterization of PTMs, but first must rely on a variety of separation techniques such as 2D gels (e.g. Halligan, et al., Nucleic Acids Res. 2004;32: W638-44) or 2D liquid chromatography to separate complex protein or peptide mixtures before mass spectral analysis is feasible. We are developing novel, designer fluorescent dyes, in collaboration with Prof. Grieco's group, that enhance detection sensitivity, pinpoint posttranslational modifications, and monitor changes in enzyme activity in the proteome. We are applying this new proteomics technology for example, to finding new diagnostic tools for type 2 diabetes, to increased understanding of immune adjuvants, to better understanding neurodevelopment, and aging in mitochondria.
Sands DC, Morris CE, Dratz EA, Pilgeram AL:
Piscitelli CL, Angel TE, Bailey BW, Hargrave P, Dratz EA, Lawrence CM. :
Riesselman M, Miettinen HM, Gripentrog JM, Lord CI, Mumey B, Dratz EA, Stie J,Taylor RM, Jesaitis AJ.:
Halligan BD, Ruotti V, Jin W, Laffoon S, Twigger SN, Dratz EA. :
Barry RC, Alsaker BL, Robison-Cox JF, Dratz EA. :
Chemistry & Biochemistry
103 Chemistry and Biochemistry Building
PO Box 173400
Bozeman, MT 59717