1. B. R. Duffus, S. Ghose, J. W. Peters, and J. B. Broderick, "Reversible H Atom Abstraction Catalyzed by the Radical SAM Enzyme HydG" J. Am. Chem. Soc. 2014, (Just Accepted). DOI: 10.1021/ja504618y
  2. J. B. Broderick, A.S. Byer, K. S. Duschene, B. R. Duffus, J. N. Betz, E. M. Shepard, and J. W. Peters, "H-Cluster assembly during maturation of the [FeFe]-hydrogenase" J. Biol. Inorg. Chem. 2014, 19, 747-757. DOI: 10.1007/s00775-014-1168-8
  3. S. Ghose, J K. Hilmer, B. Bothner, and J. B. Broderick, "Solution phase dynamics of the DNA repair enzyme spore photoproduct lyase as probed by H/D exchange" FEBS Lett. 2014, 588,3023-3029. DOI: 10.1016/j.febslet.2014.06.011
  4. E. M. Shepard, F. Mus, J. N. Betz, A. S. Byer, B. R. Duffus, J. W. Peters, and J. B. Broderick, "[FeFe]-Hydrogenase Maturation" Biochemistry 2014, 53, 4090-4104. DOI: 10.1021/bi500210x
  5. S. C. Silver, D. J. Gardenghi, S. G. Naik, E. M. Shepard, B. H. Huynh, R. K. Szilagyi, and J. B. Broderick, "Combined Mössbauer spectroscopic, multi-edge X-ray absorption spectroscopic, and density functional theoretical study of the radical SAM enzyme spore photoproduct lyase"J. Biol. Inorg. Chem. 2014, 19, 465-483. DOI: 10.1007/s00775-014-1104-y
  6. K. A. Shisler, and J. B. Broderick, "Glycyl radical activating enzymes: Structure, mechanism, and substrate interactions" Arch. Biochem. Biophys. 2014, 546, 65-71. DOI: 10.1016/j.abb.2014.01.020
  7. J. B. Broderick, B. R. Duffus, K. S. Duschene, and E. M. Shepard, "Radical S-Adenosylmethionine Enzymes" Chem. Rev. 2014, 114, 4229-4317. DOI: 10.1021/cr4004709
  8. A. V. Crain and J. B. Broderick, "Pyruvate Formate-Lyase and its Activation by Pyruvate Formate-Lyase Activating Enzyme" J. Biol. Chem. 2014, 289, 5723-5729. 10.1074/jbc.M113.496877
  9. R. C. Driesener, B. R. Duffus, E. M. Shepard, I. R. Bruzas, K. S. Duschene, N. J.-R. Coleman, A. P. G. Marrison, E. Salvadori, C. W. M. Kay, J. W. Peters, J. B. Broderick, and P. L. Roach, "Biochemical and kinetic characterization of radical AdoMet enzyme HydG" Biochemistry2013, 52, 8696-8707. DOI: 10.1021/bi401143s
  10. A. V. Crain and J. B. Broderick, "Flavodoxin cofactor binding induces structural changes that are required for protein-protein interactions with NADP+ oxidoreductase and pyruvate fomate-lyase activating enzyme" Biochim. Biophys. Acta 2013, 1834, 2512-2519. DOI: 10.1016/j.bbapap.2013.08.014
  11. D. W. Mulder, M. W. Ratzloff, E. M. Shepard, A. S. Byer, S. M. Noone, J. W. Peters, J. B. Broderick, and P. W. King, "EPR and FTIR Analysis of the Mechanism of H2 Activation by [FeFe]-Hydrogenase HydA1 from Chlamydomonas reinhardtiiJ. Am. Chem. Soc. 2013, 135,6921-6929. DOI: 10.1021/ja4000257
  12. K. A. Shisler, and J. B. Broderick, "Emerging themes in radical SAM chemistry" Curr. Opin. Struct. Biol. 2012, 22, 701-710. DOI: 10.1016/j.sbi.2012.10.005
  13. N. Joshi, E. M. Shepard, A. S. Byer, K. D. Swanson, J. B. Broderick, and J. W. Peters, "Iron-sulfur cluster coordination in the [FeFe]-hydrogenase H cluster biosynthetic factor HydF" FEBS Lett. 2012, 586, 3939-3943. DOI: 10.1016/j.febslet.2012.09.028
  14. R. U. Hutcheson and J. B. Broderick, "Radical SAM enzymes in methylation and methylthiolation" Metallomics 2012, 4, 1149-1154. DOI: 10.1039/c2mt20136d
  15. J. W. Peters and J. B. Broderick, "Emerging Paradigms for Complex Iron-Sulfur Cofactor Assembly and Insertion" Annu. Rev. Biochem. 2012, 81, 429-450. DOI: 10.1146/annurev-biochem-052610-094911
  16. B. R. Duffus, T. L. Hamilton, E. M. Shepard, E. S. Boyd, J. W. Peters, and J. B. Broderick, "Radical AdoMet enzymes in complex metal cluster biosynthesis" Biochim. Biophys. Acta 2012,1824, 1254-1263. DOI: 10.1016/j.bbapap.2012.01.002
  17. S.-H. Kim, C. Harzman, J. K. Davis, R. Hutcheson, J. B. Broderick, T. L. Marsh, and J. M. Tjedje, "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction," BMC Microbiology, 2012, 12, 21. DOI: 10.1186/1471-2180-12-21
  18. A. Dey, Y. Peng, W. E. Broderick, B. Hedman, K. O. Hodgson, J. B. Broderick, and E. I. Solomon, "S K-edge XAS and DFT Calculations on SAM Dependent Pyruvate Formate-Lyase Activating Enzyme: Nature of Interaction between the Fe4S4 Cluster and SAM and its Role in Reactivity" J. Am. Chem. Soc. 2011, 133, 18656-18662. DOI: 10.1021/ja203780t
  19. D. W. Mulder, E. M. Shepard, J. E. Meuser, N. Joshi, P. W. King, M. C. Posewitz, J. B. Broderick, and J. W. Peters "Insights into [FeFe]-Hydrogenase Structure, Mechanism, and Maturation," Structure, 2011, 19, 1038-1052. DOI: 10.1016/j.str.2011.06.008
  20. E. M. Shepard, E. S. Boyd, J. B. Broderick, and J. W. Peters, "Biosynthesis of complex iron-sulfur enzymes," Curr. Opin. Chem. Biol. 2011, 15, 319-327. DOI: 10.1016/j.cbpa.2011.02.012
  21. K. D. Swanson, B. R. Duffus, T. E. Beard, J. W. Peters, and J.B. Broderick, "Cyanide and Carbon Monoxide Ligand Formation in Hydrogenase Biosynthesis," Eur. J. Inorg. Chem.2011,935-947. DOI: 10.1002/ejic.201001056
  22. Y. Peng, S. E. Veneziano, G. D. Gillispie, and J. B. Broderick, "Pyruvate formate-lyase: Evidence for an open conformation favored in the presence of its activating enzyme," J. Biol. Chem. 2010, 285, 27224-27231. DOI: 10.1074/jbc.M109.096875
  23. E. M. Shepard, B. R. Duffus, S. J. George, S. E. McGlynn, M. R. Challand, K. D. Swanson, P. L. Roach, S. P. Cramer, J. W. Peters, and J. B. Broderick, "[FeFe]-Hydrogenase Maturation: HydG-Catalyzed Synthesis of Carbon Monoxide," J. Am. Chem. Soc. 2010, 132, 9247-9249. DOI: 10.1021/ja1012273
  24. J. B. Broderick, "A radically different enzyme," Nature 2010, 465, 877-878. DOI: 10.1038/465877a
  25. E. M. Shepard, S. E. McGlynn, A. L. Bueling, C. S. Grady-Smith, S. J. George, M. A. Winslow, S. P. Cramer, J. W. Peters, and J. B. Broderick, "Synthesis of the 2Fe subcluster on the [FeFe]-hydrogenase H cluster on the HydF scaffold," P. Natl. Acad. Sci. U.S.A. 2010, 107,10448-10453. DOI: 10.1073/pnas.1001937107
  26. D. W. Mulder, E. S. Boyd, R. Sarma, R. K. Lange, J. A. Endrizzi, J. B. Broderick, and J. W. Peters, "Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydAΔEFG," Nature 2010, 465, 248-251. (PDF)
  27. S. C. Silver, T. Chandra, E. Zilinskas, S. Ghose, W. E. Broderick, and J. B. Broderick, "Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by spore photoproduct lyase," J. Biol. Inorg. Chem. 2010, 15, 943-955. DOI: 10.1007/s00775-010-0656-8
  28. T. Chandra, W. E. Broderick, and J. B. Broderick, "An Efficient Deprotection of N-trimethylsilylethoxymethyl (SEM) Groups from Dinucleosides and Dinucleotides,"Nucleos., Nucleot., Nucl. 2010, 29, 132-143. (PDF)
  29. K. S. Duschene, and J. B. Broderick, "The antiviral protein viperin is a Radical SAM enzyme,"FEBS Lett. 2010, 584, 1263-1267. (PDF)
  30. R. C. Driesener, M. R. Challand, S. E. McGlynn, E. M. Shepard, E. S. Boyd, J. B. Broderick, J. W. Peters, and P. L. Roach, "[FeFe]-Hydrogenase Cyanide Ligands Derived from S-Adenosylmethionine-Dependent Cleavage of Tyrosine," Angew. Chem. Int. Ed. 2010, 49, 1687-1690. (PDF)
  31. S. E. McGlynn, E. S. Boyd, E. M. Shepard, R. K. Lange, R. Gerlach, J. B. Broderick, and J. W. Peters, "Identification and Characterization of a Novel Member of the Radical AdoMet Enzyme Superfamily and Implications for the Biosynthesis of the Hmd Hydrogenase Active Site Cofactor," J. Bacteriol. 2010, 192, 595-598. (PDF)
  32. T. Chandra, W. E. Broderick, and J. B. Broderick, “Chemoselective Deprotection of Triethylsilyl Ethers,” Nucleos., Nucleot., Nucl. 2009, 28, 1016-1029. (PDF)
  33. E. M. Shepard and J. B. Broderick, “S-Adenosylmethionine and iron-sulfur clusters in biological radical reactions: The radical SAM superfamily.” In Comprehensive Natural Products Chemistry II, 2009, in press.
  34. J. Yang, S. G. Naik, D. O. Ortillo, R. García-Serres, M. Li, W. E. Broderick, B. H. Huynh, and J. B. Broderick "The Iron-Sulfur Cluster of Pyruvate Formate-Lyase Activating Enzyme in Whole Cells: Cluster Interconversion and a Valence-Localized [4Fe-4S]2+ State," Biochemistry,2009, 48, 9234-9241. (PDF)
  35. K. S. Duschene, S. E. Veneziano, S. C. Silver, and J. B. Broderick, “Control of radical chemistry in the AdoMet radical enzymes, Curr. Op. Chem. Biol. 2009, 13, 74-83. (PDF)
  36. S. E. McGlynn, D. W. Mulder, E. M. Shepard, J. B. Broderick, and J. W. Peters, “Hydrogenase cluster biosynthesis: organometallic chemistry nature’s way,” Dalton Trans., 2009, 4274-4285.(PDF)
  37. T. Chandra, S. C. Silver, E. Zilinskas, E. M. Shepard, W. E. Broderick, and J. B. Broderick, “Spore Photoproduct Lyase Catalyzes Specific Repair of the 5R but not the 5S Spore Photoproduct,” J. Am. Chem. Soc.. 2009, 131, 2420-2421. (PDF)
  38. D. W. Mulder, D. O. Ortillo, D. J. Gardenghi, A. V. Naumov, S. S. Ruebush, R. K. Szilagyi, B. H. Huynh, J. B. Broderick, and J. W. Peters, “Activation of HydAΔEFG Requires a preformed [4Fe-4S] Cluster," Biochemistry, 2009, 48, 6240-6248. (PDF)
  39. J. Vey, J. Yang, M. Li, W. E. Broderick, J. B. Broderick, and C. Drennan, “Structural Basis for Glycyl Radical Formation by Pyruvate Formate-lyase Activating Enzyme,” Proc. Natl. Acad. Sci., U.S.A, 2008, 205, 16137-16141. (PDF)
  40. S. E. McGlynn, E. M. Shepard, M. A. Winslow, A. V. Naumov, K. S. Duschene, M. C. Posewitz, W. E. Broderick, J. B. Broderick, and J. W. Peters, “HydF as a Scaffold Protein in [FeFe] Hydrogenase H-cluster Biosynthesis,” FEBS Lett., 2008, 582, 2183-2187. (PDF)
  41. J. B. Broderick, “Assembling Iron-Sulfur Clusters in the Cytosol,” Nat. Chem. Biol., 2007,News & Views invited contribution, 3, 243-244. (PDF)
  42. S. E. McGlynn, S. S. Ruebush, A. Naumov, L. E. Nagy, A. Dubini, P. W. King, J. B. Broderick, M. C. Posewitz, and J. W. Peters “In vitro Activation of [FeFe] Hydrogenase: New Insights into Hydrogenase Maturation,” J. Biol. Inorg. Chem., 2007, 12, 443–447. (PDF)
  43. M. R. Nnyepi, Y. Peng, and J. B. Broderick, “On the Activation and Inactivation of Pyruvate Formate-Lyase: Role of AdhE and Small Molecules,” Arch. Bioch. Biophys., 2007, 459, 1-9.(PDF)
  44. J. M. Buis, J. Cheek, E. Kalliri, J. B. Broderick “Characterization of an Active Spore Photoproduct Lyase, an Enzyme in the Radical SAM Superfamily,” J. Biol. Chem., 2006, 381,25994-26003. (PDF)
  45. J. B. Broderick, “Fe-S Clusters in Radical Generation.” Invited contribution to Biological Inorganic Chemistry: Structure and Reactivity, I. Bertini, H. B. Gray, and J. S. Valentine, Eds., University Science Books, 2006.
  46. C. Walsby, D. Ortillo, J. Yang, M. R. Nnyepi, W. E. Broderick, B. M. Hoffman, and J. B. Broderick, “Spectroscopic Approaches to Elucidating Novel Iron-Sulfur Chemistry in the “Radical SAM” Protein Superfamily,” Inorg. Chem. 2005, 44, 727-741. (PDF)
  47. J. M. Buis and J. B. Broderick, “Pyruvate Formate-Lyase Activating Enzyme: Elucidation of a Novel Mechanism for Glycyl Radical Formation,” Arch. Bioch. Biophys. 2005, 433, 288-296.(PDF)
  48. M. M. Cosper, N. J. Cosper, W. Hong, W. E. Broderick, J. E. Shokes, J. B. Broderick, M. K. Johnson, and R. A. Scott, “The Direct Interaction between the FeS Cluster and SAM is not a Universal Feature of Radical SAM Enzymes,” Protein Sci. 2003, 12, 1573-1577. (PDF)
  49. J. B. Broderick, C. Walsby, W. E. Broderick, C. Krebs, W. Hong, D. Ortillo, J. Cheek, B. H. Huynh, and B. M. Hoffman, “Paramagnetic Resonance in Mechanistic Studies of Fe-S/Radical Enzymes, in ACS Symposium Series 858: Paramagnetic Resonance of Metallobiomolecules, J. Telser, Ed., American Chemical Society: Washington, DC, 2003. (PDF)
  50. J. B. Broderick, “Iron-Sulfur Clusters in Enzyme Catalysis.” Invited contribution toComprehensive Coordination Chemistry II: From Biology to Nanotechnology, Volume 8, L. Que and W. B. Tolman, Volume Eds., J. McCleverty and T. Meyer, Eds., Elsevier Science, 2003.(PDF)
  51. C. J. Walsby, D. Ortillo, W. E. Broderick, J. B. Broderick, B. M. Hoffman, “An Anchoring Role for FeS Clusters: Chelation of the Amino Acid Moiety of S- Adenosylmethionine to the Unique Iron Site of the [4Fe-4S] Cluster of Pyruvate Formate-Lyase Activating Enzyme,” J. Am. Chem. Soc. 2002, 124, 11270-11271. (PDF)
  52. J. Cheek and J. B. Broderick, “Direct H atom Abstraction from Spore Photoproduct C-6 Initiates DNA Repair in the Reaction Catalyzed by Spore Photoproduct Lyase: Evidence for a Reversibly Generated Adenosyl Radical Intermediate,” J. Am. Chem. Soc., 2002, 124, 2860-2861. (PDF)
  53. C. Krebs, W. E. Broderick, T. F. Henshaw, J. B. Broderick, and B. H. Huynh, “Coordination of Adenosylmethionine to a Unique Iron Site of the [4Fe-4S] of Pyruvate Formate-Lyase Activating Enzyme: A Mössbauer Spectroscopic Study,” J. Am. Chem. Soc., 2002, 124, 912-913. (PDF)
  54. C. Walsby, W. Hong, W. E. Broderick, D. Ortillo, J. B. Broderick, and B. M. Hoffman, "Electron-Nuclear Double Resonance Spectroscopic Evidence that S-Adenosylmethionine Binds in Contact With the Catalytically Active [4Fe-4S]+ Cluster of Pyruvate Formate-Lyase Activating Enzyme," J. Am. Chem. Soc., 2002, 124, 3143-3151. (PDF)
  55. J. Cheek and J. B. Broderick, “Adenosylmethionine-Dependent Iron-Sulfur Enzymes: Versatile Clusters in a Radical New Role,” J. Biol. Inorg. Chem., 2001, 6, 209-226. (PDF)
  56. C. Krebs, T. F. Henshaw, J. Cheek, B. H. Huynh, and J. B. Broderick, “Conversion of 3Fe-4S to 4Fe-4S Clusters in Native Pyruvate Formate-Lyase Activating Enzyme: Mössbauer Characterization and Implications for Mechanism,” J. Am. Chem. Soc., 2000, 122, 12497-12506. (PDF)
  57. J. R. Miller, R. W. Busby, S. W. Jordan, J. Cheek, T. F. Henshaw, G. A. Ashley, J. B. Broderick, J. E. Cronan, Jr., and M. A. Marletta, “Escherichia coli LipA is a Lipoyl Synthase: In vitro Biosynthesis of Lipoylated Pyruvate Dehydrogenase Complex from Octanoyl-Acyl Carrier Protein,” Biochemistry, 2000, 39, 15166-15178. (PDF)
  58. T. F. Henshaw, J. Cheek, and J. B. Broderick, “The [4Fe-4S]1+ of Pyruvate Formate-Lyase Activating Enzyme Generates the Glycyl Radical on Pyruvate Formate-Lyase: EPR-Detected Single Turnover,” J. Am. Chem. Soc., 2000, 122, 8331-8332. (PDF)
  59. J. B. Broderick, T. F. Henshaw, J. Cheek, K. Wojtuszewski, S. R. Smith, M. R. Trojan, R. M. McGhan, A. Kopf, M. Kibbey, and W. E. Broderick “Pyruvate formate-lyase activating enzyme: Strictly anaerobic isolation yields active enzyme containing a [3Fe-4S]+ cluster,”Biochem. Biophys. Res. Commun., 2000, 269, 451-456. (PDF)
  60. J. B. Broderick, Coenzymes and Cofactors, In: Encyclopedia of Life Sciences, Nature Publishing Group: London, www.els.net (2000).
  61. J. B. Broderick, “Catechol Dioxygenases,” Essays Biochem., 1999, 34, 173-189. (PDF)
  62. J. B. Broderick, R. A. Duderstadt, D. C. Fernandez, K. Wojtuszewski, T. F. Henshaw, and M. K. Johnson, “Pyruvate Formate-Lyase Activating Enzyme is an Iron-Sulfur Protein,” J. Am. Chem. Soc., 1997, 31, 7396-7397. (PDF)
  63. S. Booker, S. Licht, J. Broderick, and J. Stubbe, "Coenzyme B12-dependent Ribonucleotide Reductase: Evidence for the Participation of Five Cysteine Residues in Ribonucleotide Reduction," Biochemistry, 1994, 33, 12676-12685. (PDF)
  64. J. B. Broderick, M. J. Natan, T. V. O’Halloran, and R. P. Van Duyne, “Surface-Enhanced Resonance Raman Spectroscopic Studies of an Active Non-Heme Iron Enzyme Adsorbed at a Ag Surface,” Biochemistry, 1993, 32, 13771-13776. (PDF)
  65. S. Booker, J. Broderick, and J. Stubbe, "Ribonucleotide Reductases: Radical Enzymes with Suicidal Tendencies," Biochem. Soc. Trans., 1993, 21, 727-730.
  66. J. Stubbe, S. Booker, J. Broderick, S. S. Mao, M. Ator, G. Harris, G. Ashley, A .E. Linn, and G. X. Yu, "Ribonucleotide Reductases: Radical Enzymes with Suicidal Tendencies," Nucleic Acids Symp. Ser., 1993, 29, 107.
  67. J. B. Broderick and T. V. O’Halloran, “Overproduction, Purification, and Characterization of Chlorocatechol Dioxygenase, A Nonheme-Iron Dioxygenase with Broad Substrate Tolerance,”Biochemistry, 1991, 30, 7349-7358. (PDF)
  68. J. H. Cooley, E. J. Evain, R. D. Willett, and J. T. Blanchette (Broderick), "Reactions of 1,1-Dimethyl-4-Substituted Semicarbazides with Phosgene,” J. Org. Chem. 1989, 54, 1048-1051.(PDF)
  69. J. T. Blanchette (Broderick) and R. D. Willett, “Magnetic and Structural Correlations in
    [(C5H5N)NH2]2Cu2Cl6 and [(C5H5N)NH2]2Cu2Br6•H2O,” Inorg. Chem. 1988, 27, 843-849.(PDF)
  70. H. B. Krishnan, J. T. Blanchette (Broderick), and T. W. Okita, “Wheat Invertases: Characterization of Cell Wall Bound and Soluble Forms,” Plant Physiol. 1985, 78, 241-245.(PDF)