Mass Spectrometry
Proteomics and Biological Mass Spectrometer Facility
The mission of the Facility is to seed proteomics and mass spectrometry technology to research labs at Montana State University and affiliated programs. Periodic hands-on training sessions are offered by the Facility. The goal of the training modules is to expand facility access to researchers with little previous training in proteomics and mass spectrometry. Individual training can also be arranged. Please contact Dr. Brian Bothner if you wish to be trained.
The Facility has the following mass spectrometers: Bruker MALDI ToF, Bruker MicroToF-HPLC, Agilent Ion-Trap with attached ChipLC modules and a Shimadzu GCMS. The Agilent microfluidics “ChipLC” system has on-board columns, switching valves, and nano sprayer. The "ChipLC" system makes virtually all of the fluidics connections, and users have their own microfluidic chip that they "plug into" a receiver system. With exchangeable LC interfaces, most problems which hinder nanospray incorporation by relatively inexperienced users are eliminated. This technology promises to be a real breakthrough for user access to this difficult technique.
The Facility also possesses a full range of gel electrophoresis equipment including a GE Typhoon Trio (TM) variable mode scanner / imager with advanced spot evaluation software.
Mass Spectrometry Facility Instrumentation
GC
Agilent 5890Series IIplus/5971 GCMS
Ion Traps:
Bruker Esquire 3000 with Agilent 1100 HPLC (capLC), autosampler, MWD and Fraction
Collector/MALDI spotter
Agilent XCTPlus with Agilent 1100 HPLC (capLC and nanoLC) autosampler, ChipLC
TOFs:
Bruker Biflex III MALDI-TOF with High mass modifications and Scout 386 source
Bruker Microtof (ESI-TOF) with Agilent HPLC and autosampl
Mass Spectrometry and Proteomics fee Schedule
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MSU |
Non-academic |
| Walk-up Mass Spec |
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| Fees: |
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| GCMS |
$15/hour |
$30/hour |
| MALDI |
$15/hour |
$30/hour |
| XCT Ion Trap |
$50/hour |
$100/hour |
| Microtof |
$30/hour |
$60/hour |
| TBD |
TBD |
TBD |
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| Facility Run Sample |
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| Microtof High Resolution |
$30/Sample |
$60/Sample |
| Microtof Low REsolutino |
$15/Sample |
$30/Sample |
| MALDI |
$15/Sample |
$30/Sample |
| GCMS |
Inquire for Pricing |
Inquire for Pricing |
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$45 set-up incl. 1 |
$90 set-up incl. 1 |
| HPLC - Microtof |
sample, $15 ea. add. |
sample, $30 ea. |
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sample |
add. sample |
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| 2D Gel Facility Fees: |
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| Unlimited access to: |
$300/month or $1500/department/month |
| Decyder, Casters, |
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| IPGphors, |
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| Electrophoresis tanks, |
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| Typhoon |
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| Facility Run 2D gels: |
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| Single percentage gell |
Inquire for Pricing |
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| Gradient gel |
Inquire for Pricing |
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Additional fees for other strains or pre-labels apply. |
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We do not accept instrumentation/software in place of fee. |
Mass Spec-Protocols
Amersham 2D Manua
Ettan DALT II System Manual
In-Gel Digestion Protocol (preferred)
Polyacrylamide Gel Staining Protocols
Silver Staining Protocol
SyproRuby Staining Protocol
BioRad Bradford Assay Protocol
Bradfort Assay Protocol
Sample Precipitation Protocols
E. coli lysate Protocol
DRG Sample Preparation
P1 Preparation with S1
Membrane separation Protocol
Egg Yolk
Fatty Acid Extraction and Methylation
Mass Spectrometry Facility Personnel
Director:
Brian Bothner, PhD
bbothner@chemistry.montana.edu
406-994-5270
Facility Coordinator / Research Associate:
Philip Clark
pclark@chemistry.montana.edu
406-994-7297
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